Dr.Subhashis Pal

Osteoimmunology, Immuno-oncology, Bone Biology, Gut-Bone axis

Campus: SRM Medical Research Centre, Medicine & Health Sciences, Kattankulathur – Chennai

Experience: 6 years of experience

Research Interest: Our research group is interested in studying the crosstalk between immune cells and bone cells (osteoblast, osteoclast, osteocyte) in different pathophysiological conditions. We also study the tumor cells' bone metastasis process and how tumor cells can hide in the bone microenvironment by avoiding immune surveillance. The gut microbiome is another unexplored player, that can contribute largely to regulating our immune and bone health. Our lab is also interested in studying the underlying mechanism for the Gut-Bone axis.

Education:

Publication:

Key Publications:

Original research Article:

• Pal S, Morgan X, Dar H, Gacasan A, Patil S, Stoica A, Hu Y, Jones RM, Weitzmann MN, and Pacifici R. Microbiome mediated skeletal effects of gender affirming hormone therapy in mice. The Journal of Clinical Investigation. 2024 Mar 26:e175410. (IF 15.9).
• Duggal S, Sharma S, Rai N, Chauhan D, Upadhyay V, Srivastava S, Porwal K, Kulkarni C, Trivedi AK, Gayen JR, Mishra PR, Chattopadhyay N, Pal S*. Anti-microbial drug Metronidazole promotes fracture healing: enhancement of bone regenera-tive efficacy of the drug by a biodegradable sustained-release in-situ gel formulation. Biomedicines. 2024 Jul 18;12(7):1603. (IF 3.9) (*Corresponding author)
• Pal S*, Sharma S*, Porwal K, Tiwari MC, Khan Y, Kumar S, Kumar N, Chattopadhyay N. The role of osteogenic effects and vascular function in bone health in hypertensive rats: a study of anti-hypertensive and hemorheologic drugs. Calcified tissue International. Dec 15, 2023. doi: 10.1007/s00223-023-01170-4 (*Contributed equally) (IF 4.2).
• Pal S, Perrien D, Yumoto T, Faccio R, Stoica A, Coopersmith C.M., Jones R.M., Weitzmann N, and Pacifici R. The microbiome restrains melanoma bone growth by promoting intestinal NK and Th1 cells homing to bone. The Journal of Clinical Investigation. 2022 May 3;e157340. (IF 15.9).
• Yu M*, Pal S*, Paterson C, Jau-Y-Li, Tyagi A, Adams J, Coopersmith C, Weitzmann N, Pacifici R. Ovariectomy induces bone loss via microbial-dependent trafficking of intestinal TNF+ T cells and Th17 cells. The Journal of Clinical Investigation. 2021 Feb 15;131(4):e143137. (*Contributed equally) (IF 15.9).
• Pal S*, Sayeed M*, Kumar A, Verma D, Harioudh M, Verma N, Porwal K, Sharma S, Kulkarni C, Bandyopadhyay A, Mugale M, Mitra K, Ghosh JK, Chattopadhyay N. Self-Assembling Nano-Globular Peptide from Human Lactoferrin Acts as a Systemic Enhancer of Bone Regeneration: A Novel Peptide for Orthopedic Application. ACS Applied Materials and Interfaces. 2021 Apr 21;13(15):17300-17315. (IF 9.5). (*Contributed equally)
• Pal S, Singh M, Porwal K, Rajak S, Das N, Rajput S, Trivedi A.K, Maurya R, Sinha R.A., Siddiqi M.I., Sanyal S, Chattopadhyay N. Adiponectin receptors by increasing mitochondrial biogenesis and respiration promote osteoblast differentiation: discovery of isovitexin as a new class of small molecule adiponectin receptor modulator with potential osteoanabolic function. European Journal of Pharmacology. 2021 Dec 15; 913:174634. (IF 5).
• Pal S*, Sharma S*, Porwal K, Riyazuddin M, Chattopadhyay S, Sanyal S, Gayen J.R., Chattopadhyay N. Oral Administration of Isovitexin, a Naturally Occurring Apigenin Derivative Showed Osteoanabolic Effect in Ovariectomized Mice: A Comparative Study with Teriparatide. Calcified Tissue International. 2022 Apr 22. doi: 10.1007/s00223-022-00979-9. (IF 4.2). (*Contributed equally).
• Pal S, Rashid M, Singh SK, Porwal K, Singh P, Mohamed R, Gayen JR, Wahajuddin M, Chattopadhyay N. Skeletal restoration by phosphodiesterase 5 inhibitors in osteopenic mice: Evidence of osteoanabolic and osteoangiogenic effects of the drugs. Bone. 2020 Jun;135:115305. (IF 4.1).
• Pal S, Porwal K, Rajak S, Sinha RA, Chattopadhyay N. Selective dietary polyphenols induce differentiation of human osteoblasts by adiponectin receptor 1-mediated reprogramming of mitochondrial energy metabolism. Biomedicine and Pharmacotherapy. 2020 May 15;127:110207. (IF 7.5).
• Pal S, Mittapelly N, Husain A, Kushwaha S, Chattopadhyay S, Kumar P, Ramakrishna E, Kumar S, Maurya R, Sanyal S, Gayen JR, Mishra PR, Chattopadhyay N. A butanolic fraction from the standardized stem extract of Cassia occidentalis L delivered by a self-emulsifying drug delivery system protects rats from glucocorticoid-induced osteopenia and muscle atrophy. Scientific Reports. 2020 Jan 13;10(1):195. (IF 4.6).
• Pal S, Porwal K, Singh S, Malik Y, Rashid M, Kulkarni C, Khan Y, Jagavelu K, Wahajuddin M, Chattopadhyay N. Reversal of Osteopenia in Ovariectomized Rats by Pentoxifylline: Evidence of Osteogenic and Osteo‑Angiogenic Roles of the Drug. Calcified Tissue International. 2019 Sep;105(3):294-307. (IF 4.2).
• Pal S, Kumar P, Ramakrishna E, Kumar S, Porwal K, Kumar B, Arya K, Maurya R, Chattopadhyay N. Extract and fraction of Senna occidentalis L. have osteogenic effect and prevent glucocorticoid-induced osteopenia. Journal of Ethnopharmacology. 2019 May 10;235:8-18. (IF 5.4).
• Pal S, Maurya S, Chattopadhyay S, China SP, Porwal K, Kulkarni C, Sanyal S, Sinha R, Chattopadhyay N. The osteogenic effect of liraglutide involves enhanced mitochondrial biogenesis in osteoblasts. Biochemical Pharmacology. 2019 Mar 15;164:34-44. (IF 5.8).
• Pal S, Porwal K, Khanna K, Gautam M, Malik Y, Rashid M, Wahajuddin M, Parameswaran V, Bellare J, Chattopadhyay N. Oral dosing of pentoxifylline, a pan-phosphodiesterase inhibitor restores bone mass and quality in osteopenic rabbits by an osteogenic mechanism: a comparative study with human parathyroid hormone. Bone 2019 Mar 9;123:28-38. (IF 4.1).
• Bhattacharyya S*, Pal S*, Riyazuddin M, Singh P, Chattopadhyay S, China SP, Porwal K, Sanyal S, Gayen JR, Chattopadhyay N. A nutraceutical composition containing diosmin and hesperidin has osteogenic and anti-resorptive effects and expands the anabolic window of teriparatide. Biomedicine & Pharmacotherapy. 2019 Oct;118:109207. (IF 7.5). (*Contributed equally)
• China SP*, Pal S*, Chattopadhyay S, Porwal K, Mittal M, Sanyal S, Chattopadhyay N. The wakefulness promoting drug Modafinil causes adenosine receptor-mediated upregulation of receptor activator of nuclear factor κB ligand in osteoblasts: Negative impact of the drug on peak bone accrual in rats. Toxicology and Applied Pharmacology. 2018 Apr 9;348:22-31 (IF 3.8). (*Contributed equally).
• Porwal K*, Pal S*, Dev K, China SP, Kumar Y, Singh C, Barbhuyan T, Sinha N, Sanyal S, Trivedi AK, Maurya R, Chattopadhyay N. Guava fruit extract and its triterpene constituents have osteoanabolic effect: Stimulation of osteoblast differentiation by activation of mitochondrial respiration via the Wnt/β-catenin signaling. Journal of Nutritional Biochemistry. 2017 Jun; 44:22-34. (IF 5.6). (*Contributed equally)
• Pal S, Khan K, Pal China S, Mittal M, Porwal K, Shrivastava R, Taneja I, Hossain Z, Mandalapu D, Gayen J, Wahajuddin M, Sharma V, Trivedi A, Sanyal S, Bhadauria S, Godbole M, Gupta S, Chattopadhyay N. Theophylline, a methylxanthine drug induces osteopenia and alters calciotropic hormones, and prophylactic vitamin D treatment protects against these changes in rats. Toxicology and Applied Pharmacology. 2016 Mar 15;295:12-25. (IF 3.8).
• Tripathi JK*, Pal S*, Awasthi B, Kumar A, Tandon A, Mitra K, Chattopadhyay N, Ghosh JK. Variants of self-assembling peptide, KLD-12 that show both rapid fracture healing and antimicrobial properties. Biomaterials. 2015 Jul; 56:92-103. (IF 14). (*Contributed equally).
• Pal S. Editorial: Expert opinion in bone research: 2021. Frontiers in Endocrinology. 2022 Nov 16; 13:1071125. (IF 5.2).
• Acharya TK*, Pal S*, Ghosh A*, Kumar S, Kumar S, Chattopadhyay N, Goswami C. TRPV4 regulates osteoblast differentiation and mitochondrial function that are relevant for channelopathy. Frontiers in Cell and Developmental Biology. 2023 Jun 12;11:1066788. (IF 5.5). (*Contributed equally).
• Dar HY, Perrien DS, Pal S, Stoica A, Uppuganti S, Nyman JS, Jones RM, Weitzmann MN, Pacifici R. Callus γδ T cells and microbial-induced intestinal Th17 cells improve fracture healing in mice. Journal of Clinical Investigation. 2023 Apr 17;133(8):e166577. (IF 15.9).
• Li JY, Yu Mingcan, Pal S, Tyagi AM, Dar H, Adams J, Weitzmann N, Jones R, Pacifici R. Parathyroid hormone–dependent bone formation requires butyrate production by intestinal microbiota. Journal of Clinical Investigation. 2020 Apr 1;130(4):1767-1781. (IF 15.9).
• Acharya T.K, Kumar S, Tiwari N, Ghosh A, Tiwari A, Pal S, Majhi R.K, Kumar A, Das R, Singh A, Maji P.K., Chattopadhyay N, Goswami L, Goswami C. TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering. Scientific Reports 2021 Feb 12;11(1):3730. (IF 4.6).
• Tyagi A, Darby T, Hsu E, Yu M, Pal S, Dar H, Jau-Yi Li, Adams J, Jones R, and Pacifici R. The gut microbiota is a transmissible determinant of skeletal maturation. eLIFE. 2020;9: e64237. DOI: https://doi.org/10.7554/eLife.64237. (IF 7.7).

Review Papers:

• Porwal K*, Pal S*, Bhagwati S, Siddiqi M.I., Chattopadhyay N. Therapeutic potential of phosphodiesterase inhibitors in the treatment of osteoporosis: Scopes for therapeutic repurposing and discovery of new oral osteoanabolic drugs. European Journal of Pharmacology. 2021 May; 899:174015. (IF ). (*Contributed equally)
• Bhattacharyya S, Pal S, Chattopadhyay N. Targeted inhibition of sclerostin for post-menopausal osteoporosis therapy: A critical assessment of the mechanism of action. European Journal of Pharmacology 2018 May 5;826:39-47. (IF ).
• Bhattacharyya S, Pal S, Chattopadhyay N. Abaloparatide, the second generation osteoanabolic drug: molecular mechanisms underlying its advantages over the first-in-class teriparatide. Biochemical Pharmacology. 2019 Aug; 166:185-191. (IF ).

Patents:

1. Naibedya Chattopadhyay, Subhashis Pal, Sudhir Kumar, Ramakrishna Eppalapally, Padam Kumar, Sapana, Jiaur Rahaman Gayen, Riyazuddin Mohammed, Sabyasachi Sanyal, Anagha Gurjar, Prabhat Ranjan Mishra, Naresh Mittapelly, Kamal Ram Arya, Brijesh Kumar, Srikanta Rath, Arun Kumar Trivedi, Rakesh Maurya. BIOACTIVE EXTRACT, FRACTION AND FORMULATION OF CASSIA OCCIDENTALIS FOR BONE REGENERATION AND THE PREVENTION OR TREATMENT OF GLUCOCORTICOID-INDUCED OSTEOPOROSIS AND MUSCULAR ATROPHY (US11304927B2).

Book Chapter:

1. Phytochemistry of Plants of Genus Cassia. Brijesh Kumar, Vikas Bajpai, Vikaskumar Gond, Subhashis Pal, Naibedya Chattopadhyay. CRC Press 2021, Doi: 10.1201/9781003186281.

Awards & Achievements:

Key Academic Awards:

1. Best Poster Award-Winship Scientific Symposium-2023; Winship cancer research Institute, Emory University, USA
2. Best Poster Award-Basic Science Research-2023; Emory University School of Medicine, USA
3. High Scoring Abstract Award-2023; Cancer & Bone Society (CABS), USA
4. ASBMR President's Award -2022; The American Society Bone and Mineral Research, USA
5. Best PhD Thesis Award-2019; Central Drug Research Institute, India
6. East-Meets-West early carrier Award-2018; The European Calcified Tissue Society, Belgium
7. Travel grant Award to attend ECTS-2017 conference; Science and Engineering research Board (SERB), India
8. Best paper Award-2015, 2022; Central Drug Research Institute, India
9. Senior Research Fellowship 2015-2017; The Council of Scientific and Industrial Research, India
10. Junior Research Fellowship 2013-2014; The Council of Scientific and Industrial Research, India

Workshops/Seminars/Conferences:

Conference/Symposium/workshops

• Immunocon-2024 annual meeting organized by Indian Immunology Society and Indian Institute of Science (IISc), October 2024, Bengaluru, India.
• The American society of bone and mineral research (ASBMR) 2024 Annual Meeting organized by American Society for Bone and Mineral Research, September 2024, Toronto, ON, Canada.
• “Future Proof your Career: AI & Experimental Designs for Evidence based health Research”; Faculty development workshop organized by School of Public Health, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur, India.
• The American society of bone and mineral research (ASBMR) 2023 Annual Meeting organized by American Society for Bone and Mineral Research, October 2023, Vancouver, BC, Canada.
• Emory University School of Medicine Research Day, November 2023, Atlanta, GA, USA.
• Winship Scientific Symposium 2023, Emory University, November 2023, Atlanta, GA, USA.
• Cancer and Bone Society (CABS) 2023 annual meeting, June 2023, St. Louis, Missouri, USA.
• The American society of bone and mineral research (ASBMR) 2022 annual meeting, September 2022, Austin, Texas, USA.
• The American society of bone and mineral research (ASBMR) 2021 annual meeting, September 2021, San Diego, California, USA.
• The American society of bone and mineral research (ASBMR) 2020 annual meeting, September 2020, Seattle, Washington, USA.
• European calcified tissue society (ECTS) congress, May 2018, Valencia, Spain.
• European calcified tissue society (ECTS) congress, May 2017, Salzburg, Austria.
• Indian Society for Bone & Mineral Research (ISBMR), September 2016, Mumbai, India.
• 2nd International meet on advanced studies on cell signaling network (CeSiN 2014), December 2014, Kolkata, India.

Oral Presentations:

• PTHrP Induces Breast Cancer Bone Metastasis via Gut Microbiome Dependent Expansion of TNF and IL-17 Producing T Cells. The American society of bone and mineral research (ASBMR) 2023 annual meeting, October 2023, Vancouver, BC, Canada.
• Microbiome-Induced intestinal Th17 cells and Musculoskeletal γδ Cells improve fracture healing. The American society of bone and mineral research (ASBMR) 2023 annual meeting, October 2023, Vancouver, BC, Canada.
• The Gut Microbiome restrains melanoma bone growth by promoting the homing of intestinal NK and Th1 cells to the bone marrow. The American society of bone and mineral research (ASBMR) 2022 annual meeting, September 2022, Austin, Texas, USA.
• LP4, a lactoferrin-derived small peptide reverses osteopenia via BMP2-OPG dependent pathway. European calcified tissue society congress, May 2018, Valencia, Spain.

Poster Presentations

• The Gut Microbiome restrains melanoma bone growth via gut resident immune cells. Immunocon-2024 annual conference, October 2024, Bengaluru, India.
• Gender Affirming Hormone Therapy does not impair skeletal maturation in young mice and regulates Bone via the Gut Microbiome. Emory University School of Medicine Research Day, November 2023, Atlanta, GA, USA.
• The Gut Microbiome restrains melanoma bone growth by promoting the homing of intestinal NK and Th1 cells to the bone marrow. Winship Scientific Symposium 2023, November 2023, Atlanta, GA, USA.
• Gender Affirming Hormone Therapy does not impair skeletal maturation in young mice and regulates Bone via the Gut Microbiome. The American society of bone and mineral research (ASBMR) 2023 annual meeting, October 2023, Vancouver, BC, Canada.
• Homing of intestinal NK and Th1 cells to the tumor site restrains melanoma bone growth in a gut microbiome dependent manner. Cancer and Bone Society (CABS) 2023 annual meeting, June 2023, St. Louis, Missouri, USA.
• Skeletal restoration by Liraglutide in ovariectomized rats by an osteoanabolic mode: a comparative study with PTH and Alendronate. European calcified tissue society congress, May 2017, Salzburg, Austria.
• Liraglutide restores trabecular bones in ovariectomized rats by osteoanabolic mode. Indian Society for Bone & Mineral Research (ISBMR), September 2016, Mumbai, India.
• Theophylline is the most potent among methylxanthine group of drugs in causing osteoblast cytotoxicity and induces osteopenia in rats. 2nd International meet on advanced studies on cell signaling network (CeSiN 2014), December 2014, Kolkata, India.

Work Experience:

Research Experience:

• Assistant Professor; Faculty of Medical & Health Sciences, SRM Institute of Sciences & Technology, Kattankulathur – 603202, Tamil Nādu, India. (2024-Present).
• Associate Scientist; Division of Endocrinology, Metabolism, and Lipids; Emory University, Atlanta, USA. (2023-2024).
• Post Doctoral Researcher; Division of Endocrinology, Metabolism, and Lipids; Emory University, Atlanta, USA. (2019-2023).
• Post Doctoral Researcher; Division of Endocrinology, Central Drug Research Institute, Lucknow, Uttar Pradesh, India. (2018-2019).

Teaching Experience:

• Guest Lecturer; Rammohan college, Calcutta University. (2011-2012).

Memberships:

Academic Memberships:

• American Society Bone and Mineral Research (ASBMR)
• Cancer & Bone Society (CABS).
• European Calcified Tissue Society (ECTS)

Responsibilities Held:

Editorial board member:

• BMC Molecular and Cell Biology
• Frontiers in Endocrinology
• Frontiers in Immunology
• Heliyon

Reviewer’s Role:

Biochemical Pharmacology; Biomedicine and Pharmacotherapy; Life Science; Free Radical Biology and Medicine; Biomaterials; Chemico Biological Interaction; eLife; Journal of Inflammation research; Bone; Bone Reports; Frontiers Oncology; Journal of Ethnopharmacology; Molecular and Cellular Endocrinology; Frontiers Pharmacology; Cytokine; Clinical Intervention in Aging; Gut Microbes; Drug Design; Development and Therapy; Journal of Nutritional Biochemistry; Stem cells Reviews and Reports; BioFactors; Diabetes, Metabolic syndrome and Obesity; Metabolism; International Journal of General Medicine; European Journal of Pharmacology; PeerJ; Food and Function; Behavioural Brain Research; Journal of Visualized Experiments (JOVE); Expert Opinion on Biological Therapy; Annals of Medicine.